Figure 6.

Lactose prevents staining artifacts due to partial drying of sections during staining. Two lymph node serial sections were immunostained for CD3 after both were tilted with an angle of about 15° (see scheme on top for direction of tilting). (A) Section incubated with regular antibody diluent; (B) section incubated with antibody diluent plus 10% lactose. Note the loss of staining on the anti-gravitational end of the section in (B). Original magnification. Whole slide image 1×; Scale, 1 mm. Light hematoxylin nuclear counterstain.