Figure 9.

Protective effect of lactose on the ability to erase bound antibody layers. A kidney section was immunostained for keratins (AE-1 + AE-3) with a mouse antibody (overnight), goat anti-mouse-FITC, mouse antibody, goat anti-mouse-FITC antibody sequence followed by anti-FITC HRP conjugate and DAB development (A). Before the anti-FITC conjugate, the sections were air dried in TBS (B) or TBS with 10% lactose (C), rehydrated, and stripped with 2-ME/SDS. Note the residual keratin staining in (B). Original magnification, 8×; Scale, 100 µm. Light hematoxylin nuclear counterstain.