Figure 1.

Detectable levels of sclerostin immunoreactivity (sclerostin-IR) are present in osteocyte cell bodies, osteocyte dendrites/canaliculi, and the periosteum in the cortical bone of the mouse femur. (A) Low power magnification, medial-lateral view of an H&E section of a young (4-month-old) male mouse femur. Boxed region indicates the mid-diaphyseal region where images in Figs. 1B, 1C, 2 and 3 were obtained. (B) Representative mid-power confocal image showing sclerostin-IR (fire red) in the osteocyte cell bodies and periosteum. (C) High-power confocal image showing sclerostin-IR within an osteocyte cell body (arrowhead) and the dendrite/canaliculi process (arrow). Scale (B) 25 µm; (C) 10 µm.