Figure 1.

Trabecular morphology of the MMA-embedded femur sample. Femurs from mice were processed in MMA and stained by Villanueva Osteochrome. Phase-contrast images of the samples show osteocytes (OT) represented as dark brown dense osteoid seams of trabecular bone (TB; green) and osteoblasts or lining cells (LC) of the marrow cavity (MC; light red). Sections were imaged in the cancellous or TB of the mouse femur around the MC where the LC or the active osteoblasts reside alongside of OTs (indicated by arrows). Images were taken using 20× magnification. Scale, 50 µm. Imaging was conducted using a Nikon TMS (model TMS-F #211153).