Figure 1.

CD34. (A) RT-qPCR confirmed the presence of CD34 mRNA in comparison to 18S rRNA; n=10. (B) Representative western blots. Left panel: Recombinant truncated V5-tagged CD34 was expressed in E. coli and lysates probed on the same blot with indicated antibodies. The negative control samples were collected from E. coli transfected with an empty vector without the gene fragment of interest. Anti-V5 and anti-CD34 antibodies detected the same band of about 50 kDa in size (calculated expected size: 51 kDa). Right panel: Dog skin lysates incubated with anti-CD34 antibody revealed the expected full-length protein band above 100 kDa, which was not detected when the anti-CD34 antibody was omitted. Note that both western blotting analyses were performed on the same membrane which was cut in half for incubations, as indicated with the dashed line. (C) Schematic drawing and immunohistochemical staining for CD34. Picture on the left: Anagen HF with positive staining in the ORS of the isthmus and the upper suprabulbar region. Picture in the middle: Magnification of the picture on the left. Note the membranous and cytoplasmic staining, which is most intense in the upper isthmus. Picture on the right: Telogen HF with fainter but positive staining in the ORS of the lower isthmus; n=5. Note that, for a better overview, the schematic drawings of the hair follicles depict only one follicle of each cycle stage in which a positive staining was observed. Scale (C, left) 100 µm; (C, middle) 25 µm; (C, right) 50 µm.