Figure 4.

LGR5. (A) RT-qPCR confirmed the presence of LGR5 mRNA in comparison to 18S rRNA; n=10. (B) Representative western blots. Note that we failed to generate recombinant LGR5 protein. Dog skin lysates probed with the anti-LGR5 antibody show several bands including a band of the expected size (94 kDa), which was not detected with the secondary antibody alone. Procedures were as per those specified in Fig. 1. (C) Schematic drawing and immunohistochemical staining for LGR5. Picture on the left: Late anagen HF. The cells did not show any positive staining in the entire HF. Picture in the middle: Telogen HF with positive staining of individual cells in the secondary hair germ. Note the cytoplasmic staining. Picture on the right: HF in the late telogen to anagen HC stage with positive individual cells in the secondary germ; n=5. Note that, for a better overview, the schematic drawings of the hair follicles depict only one follicle of each cycle stage in which a positive staining was observed. Scale (C, left) 100 µm; (C, middle) 25 µm; (C, right) 100 µm.