Normalized absorption spectra of Az-AuNP solutions at [AuNP]=1.02 nM and various Az concentrations: 0 µM (continuous black line), 0.7 µM (dotted black line), 2.8 µM (dashed black line), 11 µM (continuous gray line). Inset: Zoom of the wavelength region of plasmon resonance band peaks. Reprinted from Biophysical Chemistry, 2009, 139, 1–7, I. Delfino and S. Cannistraro, Optical investigation of the electron transfer protein azurin-gold nanoparticle system, Copyright (2009), with permission from Elsevier.