Virulence of Flavobacterium columnare in experimentally infected zebra fish (Danio rerio). (a) The mean longevity (±s.e., open squares, left axis) and survival percentage (filled circles, right axis) of the infected zebra fish (n = 10, in each bacterial exposure) after exposure to bacterial strains isolated in 2003–2010. (b) Survival percentage of zebra fish (n = 14 in each bacterial exposure) after exposure to bacterial strains isolated from inlet water (B355–B407) and outlet water (B399–B397) of a fish farm (filled circles for individual strains, open circles for mean survival ± s.e.).