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Culture and morphology of mouse NSCs.
(A, B) Dissection of fetal mouse brain (A) and hippocampus (B) under a dissecting microscope. (C–E) NSCs at 1 day of culture (× 100; C), showing small neurospheres and a high refractive index; 3 days of culture (× 100; D), showing enlarged neurospheres, distinct boundaries, but indistinct processes; 5 days of culture (× 100; E), showing even larger neurospheres and confluence, with a lower refractive index. NSCs: Neural stem cells.
