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. 2016 Feb;11(2):332–337. doi: 10.4103/1673-5374.177744

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Effects of high tau protein concentration on targeted migration of NSCs in the injured spinal cord of rats (Transwell assay).

(A) Cresyl violet staining of NSCs in the control group and at different tau concentrations (0, 0.1, 0.5, 1.0 mg/mL) in the lower chamber (light microscope, × 100). (B) Cresyl violet staining of NSCs in the control, OA, C2-ceramide and unpretreated NSC groups (light microscope, × 100). (C) Optical density of cresyl violet staining of NSCs in the control group and at different tau concentrations (0, 0.1, 0.5, 1.0 mg/mL) in the lower chamber (no significant differences observed between tau concentrations). (D) Optical density of cresyl violet staining of NSCs in the control group, OA group, C2-ceramide group and unpretreated NSCs group (optical density was significantly lower in the OA and C2-ceramide groups than in the unpretreated NSCs group). *P < 0.05, vs. OA group and C2-ceramide group (mean, n = 3, one-way analysis of variance and Tukey-Kramer test were used). NSCs: Neural stem cells; OA: okadaic acid; OD: optical density.