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Using NMR to confirm chemokine folding. A) 1H-15N HSQC spectra of CCL28 and CXCL12 with cysteines fully oxidized and properly paired (top row) or fully reduced using DTT (bottom row). One-dimensional 1H spectra of CCL28 (B) and CXCL12 (C) with correct disulfide parings (top) or fully reduced using DTT (bottom).
