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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Methods Enzymol. 2015 Nov 14;570:539–565. doi: 10.1016/bs.mie.2015.09.031

Table 1.

Reverse Phase HPLC gradient scheme

Time (minutes) % RP-HPLC buffer A % RP-HPLC buffer B
0 90 10
5 70 30
35 40 60
40 0 100
45 0 100
50 90 10
65 90 10

RP-HPLC buffer A: Aqueous 0.1% triflouroacetic acid

RP-HPLC buffer B: Aqueous 0.1% triflouroacetic acid, 70% acetonitrile