Table 1.
Primer pairs for target and housekeeping genes for quantitative RT-PCR assay.
| Target gene accession |
Forward | Sequence Reverse |
Probe length |
|---|---|---|---|
| P16 NM_001040654.1 |
TGCTCAACTACGGT GCAGATTC |
ATGTCTTGATGTCCC CGCTCT |
190 bp |
| P21 NM_001111099.1 |
CCAATCCTGGTGAT GTCCGA |
AGTCAAAGTTCCACC GTTCTCG |
150 bp |
| CyclinD1 NM_007631.2 |
GTGAGGAGCAGAA GTGCGAAGA |
GGCCGGATAGAGTTG TCAGTGTAG |
199 bp |
| SirT1 NM_001159589 |
AGGGAACCTTTGCC TCATCTAC |
GTTTGGCATATTCACC ACCTAGC |
103 bp |
|
β-actin NM_007393.4 |
AGATTACTGCTCTG GCTCCTAGC |
ACTCATCGTACTCCTGCT TGCT |
147 bp |