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Characteristics of bone marrow mast cells and their exosomes. (a) Bone marrow mast cells showed abundant purple granules following toluidine blue staining (400x). (b) Mast cells were positive for CD117 and IgE receptor (FcεRI) by flow cytometry analysis. (c) BMMC-exosomes displayed a cup-shape morphology; scale bar = 100 nm. (d) Western blot indicated that BMMC-exosomes expressed CD63, a marker constitutively expressed by exosomes.
