Figure 3.

Effects of BMMC-exosomes on naive CD4⁺ T cells. (a) 10⁶ naive CD4⁺ T cells were coincubated with BMMC-exosomes generating 10⁶ or 10⁷ BMMCs under Th2 conditions. Compared with the blank control without exosomes, groups containing different levels of exosomes showed higher proliferation. T cell proliferation was quantified by incorporation of CCK-8. Results are expressed as mean O.D. at 450 nm and are representative of three experiments. ∗, compared with control, p < 0.05. ∗∗, compared with 1 : 1 group, p > 0.05. Student-Newman-Keuls (SNK) test was used. (b–d) After 72 h of culturing, the exosome group showed over 28% of Th2 cells, which was about twice the control group. (e) The rate of Th2 cells in the T cells was expressed as mean ± SD ∗, compared with blank control, p < 0.05. The results represented four independent experiments.