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. 2016 Mar 21;57(3):1317–1326. doi: 10.1167/iovs.15-18958

Figure 2.

Figure 2

Cyclic GMP production in primary cultures of HTM cells stimulated by IWP-953 in the presence of DETA-NO and by DETA-NO alone. Myofibroblast-like HTM120 (A) and HTM123 (B) cultures and endothelial-like HTM130 (C) and HTM141 (D) cell cultures were seeded at a density of 1 × 105 cells/well in 24-well plates and incubated with DMEM containing IBMX (0.5 mM) for 15 minutes at 37°C. Human trabecular meshwork cell cultures were then incubated with increasing concentrations of IWP-953 ranging from 0.1 μM to 10 μM in the presence of a fixed concentration of DETA-NO (10 μM) or vehicle (0.5% DMSO), or DETA-NO (10 μM, 100 μM) for 15 minutes at 37°C. Following cell lysis, cleared HTM cell supernatants were acetylated and the amount of cGMP was measured using a cGMP enzyme immunoassay. Data are expressed as the mean ± SD (N = 3; except for HTM120, HTM130 cultures at IWP-953 [0.1 μM] with DETA-NO [10 μM]: N = 1). *P < 0.05; **P < 0.01, ***P < 0.001.