Figure 3.

Nonpolarized RPE cells and polarized RPE monolayers expressed HN receptors. Expression of CNTFRα, gp130, and WSX1 in nonpolarized (A–C) and polarized RPE cells (D–F). Nuclear staining was performed with DAPI (blue). CNTFRα and WSX1 mostly localized in the cytoplasmic compartments, while gp130 showed nuclear as well as cytoplasmic localization. However, in polarized RPE monolayers, all the proteins were found predominately in the cytoplasm. The x–z cross-section stack image demonstrated that CNTFRα and gp130 are expressed on the apical side, while WSX1 is expressed in apical as well as basal domains. Scale bar: 20 μm.