Table 2.
Age-independent accumulation of specific miRNAs in hemolymph.1
| miRNA2 | PRECURSOR COORDINATES3 | miRDeep2 SCORE4 | CPM5 | YOUNG | OLD | ||
|---|---|---|---|---|---|---|---|
| FC6 | FDR6 | FC6 | FDR6 | ||||
| dme-let-7-5p | chr2L:18472043.18472103:+ | 8440.8 | 5667.9 | 2.3 | 0.003 | 4.8 | 0.000 |
| dme-miR-11-5p | chr3R:21622502.21622563:− | 31989.8 | 18231.9 | 2.1 | 0.003 | 1.9 | 0.000 |
| dme-miR-125-5p | chr2L:18472342.18472402:+ | 3020.1 | 1751.3 | 2.4 | 0.011 | 3.4 | 0.000 |
| dme-miR-190-5p | chr3L:8571821.8571884:+ | 1852.9 | 1197.1 | 2.4 | 0.001 | 3.1 | 0.000 |
| dme-miR-252-5p | chr3R:13464230.13464296:− | 2716.2 | 1777.4 | 1.9 | 0.011 | 2.4 | 0.000 |
| dme-miR-276a-5p | chr3L:10365243.10365302:+ | 115850.2 | 64277.3 | 2.7 | 0.000 | 1.7 | 0.004 |
| dme-miR-277-5p | chr3R:10100038.10100101:+ | 24458.6 | 8898.9 | 2.3 | 0.003 | 1.6 | 0.011 |
| dme-miR-278-5p | chr2R:15657082.15657142:+ | 5465.5 | 1467.1 | 2.7 | 0.000 | 2.1 | 0.000 |
| dme-miR-279-5p | chr3R:29215605.29215670:+ | 4.8 | 3259.6 | 5.3 | 0.000 | 2.6 | 0.000 |
| dme-miR-282-5p | chr3L:3251037.3251102:+ | 10668.9 | 5833.4 | 2.7 | 0.009 | 11.6 | 0.000 |
| dme-miR-33-5p | chr3L:19797985.19798046:+ | 27140.1 | 18409.2 | 1.8 | 0.030 | 2.2 | 0.000 |
| dme-miR-34-5p | chr3R:10100951.10101025:+ | 20436.6 | 5104.1 | 2.3 | 0.007 | 3.4 | 0.000 |
| dme-miR-8-5p | chr2R:16831446.16831506:+ | 309506.2 | 187622.4 | 2.3 | 0.001 | 2.4 | 0.000 |
| dme-miR-929-5p | chr3R:4295384.4295439:+ | 439.6 | 298.7 | 6.9 | 0.000 | 4.2 | 0.000 |
| dme-miR-970-5p | chrX:12636351.12636416:+ | 1864.8 | 971.3 | 1.7 | 0.027 | 1.8 | 0.000 |
| dme-miR-980-5p | chrX:280188.280254:− | 729 | 412.3 | 17.5 | 0.000 | 21.3 | 0.000 |
| dme-miR-986-5p | chr2R:8444999.8445067:+ | 2392.6 | 1628.6 | 2.2 | 0.001 | 1.8 | 0.000 |
| dme-miR-996-5p | chr3R:29217201.29217265:+ | 4302.4 | 2324.8 | 3.7 | 0.000 | 1.8 | 0.000 |
| chr3L_16646 | chr3L:24539748.24539815:− | 1.1 | 17.3 | 8.9 | 0.023 | 14.6 | 0.000 |
| chr3R_20493 | chr3R:24268348.24268396:+ | 3.4 | 216.0 | 77.8 | 0.000 | 4.0 | 0.002 |
| chrX_28055 | chrX:7302769.7302827:+ | 1.5 | 943.8 | 349.1 | 0.000 | 113.6 | 0.000 |
| chrX_28088 | chrX:8026969.8027019:+ | 1.3 | 4856.6 | 19.5 | 0.000 | 56.6 | 0.000 |
| chrX_32979 | chrX:22373683.22373732:− | 1.3 | 2790.3 | 5.0 | 0.001 | 10.4 | 0.000 |
| chrX_33619 | chrX:23526522.23526569:− | 1557.8 | 2790.3 | 5.0 | 0.001 | 10.4 | 0.000 |
Notes:
miRNAs were considered enriched if FC ≥ 1.5 and FDR < 0.05 between HL and BT.
Names of known miRNAs are from miRBase v.21 (http://www.mirbase.org/). Novel miRNAs are labeled with a unique identification containing the chromosome and an arbitrary number assigned to the hairpin predicted by miRDeep2.
Location of the miRNA precursor in the UCSC Genome Browser dm6 (NCBI genome/47) Drosophila melanogaster genome.
The miRDeep2 score represents the log-odds probability of a sequence being genuine miRNA precursor versus the probability that it is a background hairpin.
Average miRNA read counts-per-million computed over all libraries and taking into account the estimated dispersions and the libraries sizes. It represents a measure of the overall expression level of the miRNA.
Fold change and FDR values for differential expression were computed by EdgeR from pairwise comparisons between HL and BT to identify the miRNAs that are enriched in the hemolymph at young and old ages.