Table 1.
List of antibodies and other markers, including their working dilution, used in this study
| Primary antibodies (Millipore) | Secondary antibodies (Invitrogen) | Other markers (Sigma1; Invitrogen2) |
|---|---|---|
| Goat anti-choline acetyltransferase (1:100) | Rabbit anti-goat Alexa Fluor 488 (1:500/1:300) | Phalloidin Atto647 (1:100)1 |
| Chicken anti-neurofilament (1:500/1:300) | Donkey anti-goat Alexa Fluor 568 (1:500/1:300) | α-bungarotoxin rhodamine/Alexa Fluor 488 (1:500/1:300)1 |
| Rabbit anti-CGRP (1:400/1:300) | Goat anti-chicken Alexa Fluor 568 (1:500/1:300) | Avidin Alexa Fluor 488 (1:250/1:150)2 |
| Mouse anti-synaptophysin (1:500/1:200) | Goat anti-rabbit Alexa Fluor 488 (1:500/1:300) | Streptavidin Alexa Fluor 568 (1:500/1:300)2 |
| Goat anti-mouse Alexa Fluor 488 (1:500/1:300) | Streptavidin-HRP (1:1000/1:600)2 |
The first working dilution within the bracket is for the staining of sections of the muscle belly, the second working dilution is for staining of the whole mounts.