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. Author manuscript; available in PMC: 2016 Mar 29.
Published in final edited form as: Innate Immun. 2013 Sep 17;20(6):606–617. doi: 10.1177/1753425913503386

Fig. 2.

Fig. 2

Pseudomonas aeruginosa AHLs stimulate mouse nasal calcium signaling that also requires components of taste signaling. (A–B) Average traces showing calcium responses in Wt cultures stimulated with 100 μM C12HSL (A; n = 3 cultures) or C4HSL (B; n = 5 cultures). (C–D) Traces showing calcium responses to C4HSL in TRPM5 knockout (TRPM5−/−; C, n = 7) and α-gustducin knockout (α-gustducin−/−; D, n = 3) ALIs. (D–E) Traces showing C4HSL-induced calcium responses in Wt cultures treated with the PLCβ2 inhibitor U73122 (D, n = 4) and the inactive U73343 (E, n = 4). (F) Bar graph showing calcium response after 5 min stimulation with C4HSL. Fluo-4 F/Fo values (mean ± SEM) after 5 min stimulation were 1.32 ± 0.07 (C12HSL; Wt), 1.25 ± 0.05 (C4HSL; Wt), 1.08 ± 0.02 (C4HSL; TRPM5−/−), 1.25 ± 0.07 (C4HSL; gustducin−/−), 1.07 ± 0.05 (C4HSL + U73122; Wt), and 1.22 ± 0.05 (C4HSL ± U73343). (H–I) To confirm the lack of role for α-gustducin, cultures from Wt (n = 5) and α-gustducin−/− (n = 5) mice were also stimulated with 1 mM PTC. (J) Bar graph showing calcium response after 5 min stimulation with PTC and peak calcium response after stimulation with ATP. Fluo-4 F/Fo values (mean ± SEM) after PTC were 1.26 ± 0.05 (Wt) and 1.24 ± 0.04 (α-gustducin−/−). Fluo-4 F/Fo values (mean ± SEM) after ATP were 2.45 ± 0.25 (Wt) and 2.68 ± 0.49 (α-gustducin−/−). Symbols denote significance determined by 1-way ANOVA with Dunnett’s (G) or Bonferroni (J) post-tests; *P <0.05, n.s. not significant.