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The RTKN stable knock-down A549 and SPC-A-1 lung adenocarcinoma cells lines. A549 and SPC-A-1 cells were transfected with lentivirus containing three constructs of RTKN shRNA (sh1, sh2, and sh3) or non-target controls shRNA (NC). Cells without transfection were used as control (CON). (A) RTKN mRNA levels were determined by RT-PCR. (B) RTKN protein levels were examined by western blotting. ***P<0.001.
