Figure 2.

Metformin (Met) inhibits cell proliferation and expression of ERα in MCF-7 and TR MCF-7 cells. (A) Cells were treated with Met at a concentration of 15, 20 and 25 mM for 72 h in each culture medium supplemented with 3% FBS. Cell proliferation was measured by cell counting using trypan blue staining. (B) The expression levels of ERα, phospho(p)-AMPKα (Thr172) and total AMPKα were examined by western blotting. β-actin was evaluated as a loading control. All data represent the mean ± SD of three independent experiments conducted in triplicates. P-value was calculated compared to the untreated Ctrl cells; ^*P<0.05 and ^**P<0.01.