Fig 3. TCR-induced proximal signaling is decreased in Gimap5 deficient T cells.

(A) Purified CD4⁺ T cells from control and Gimap5^sph/sph mice were left un-stimulated or stimulated with 5 μg/μL anti-CD3 or anti-CD3&CD28 antibodies for the indicated duration. Lysates were subjected to Western blot analysis. Representative data from one of 3 independent experiments are shown. (B) CD4⁺ T cells from OT-II TCR-transgenic control and from Gimap5^sph/sph mice were co-cultured with irradiated APC pulsed with OVA peptide at a ratio of APC: CD4⁺ T cells (8:1) for different durations of time. Cell lysates were prepared and phosphorylation of the tyrosine recidue was detected using 4G10 antibody. Representative data from one of 3 independent experiments are shown.