Skip to main content
. 2016 Mar 29;12(3):e1005917. doi: 10.1371/journal.pgen.1005917

Fig 4. PAR-1 stabilizes less phosphorylated forms of tau (taulower) through phosphorylation at Ser262 and Ser356.

Fig 4

(A) PAR-1 knockdown reduces the levels of tau with more prominent effect on taulower. Western blots of fly heads expressing tau (tau) or that co-expressing tau and PAR-1RNAi (tau+PAR-1RNAi) with anti-tau antibody. (B) PAR-1 overexpression increases the levels of tau with more prominent effect on taulower. Western blots of fly heads expressing tau (tau) or that co-expressing tau and PAR-1 (tau+PAR-1OE) with anti-tau antibody. (C) PAR-1 knockdown does not affect S2Atau levels. Western blots of fly heads expressing S2Atau (S2A) or that co-expressing S2Atau and PAR-1RNAi (S2A+PAR-1RNAi) with anti-tau antibody. (D) PAR-1 overexpression does not affect S2Atau levels. PAR-1 knockdown does not affect S2Atau levels. Western blots of fly heads expressing S2Atau (S2A) or that co-expressing S2Atau and PAR-1 (S2A+PAR-1OE) with anti-tau antibody. Tubulin or actin was used as loading control. Mean ± SD, n = 4–5; *, p < 0.05, ***, p < 0.005, n.s., not significant (p > 0.05) by Student's t-test. Representative blots are shown. Transgene expression was driven by gmr-GAL4.