Skip to main content
. 2016 Mar 29;12(3):e1005917. doi: 10.1371/journal.pgen.1005917

Fig 7. Stabilization of tau through phosphorylation at Ser262 and Ser356 contributes to the Aβ42-induced increase in the level of tau phosphorylated at Thr231.

Fig 7

(A) Aβ42 does not increase the levels of S2Atau phosphorylated at Thr231. Western blots of fly heads expressing S2Atau (S2A) or that co-expressing S2Atau and Aβ42 (S2A+Aβ42) with antibodies that recognize phosphorylation status of tau at the specific sites (pThr231, pSer202, and TAU-1) or pan-tau antibodies (tauC and tau46). (B) Aβ42 does not increase the levels of tau phosphorylated at Thr231 in the PAR-1 knockdown background. Western blots of fly heads expressing PAR-1RNAi and tau (PAR-1RNAi+tau) or that co-expressing PAR-1RNAi, tau and Aβ42 (PAR-1RNAi+tau+Aβ42) with anti-phospho-Thr231 antibody (pThr231) or pan-tau antibody (tauC). (C) PAR-1 overexpression increases the levels of tau phosphorylated at Thr231. Western blots of fly heads expressing tau (tau) or that co-expressing PAR-1 and tau (tau+PAR-1OE) with anti-phospho-Ser262 antibody (pSer262), TAU-1 or anti-phospho-Thr231 antibody (pThr231). Actin was used as loading control. Mean ± SD, n = 5; **, p < 0.01, ***, p < 0.005. Representative blots are shown. Transgene expression was driven by gmr-GAL4.