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. 2016 Mar 29;11(3):e0152282. doi: 10.1371/journal.pone.0152282

Fig 6. Affinity analysis of TAP-derived recombinant antibodies.

Fig 6

Heavy and light chain TAP fragments were transiently co-transfected into Expi293 using ExpiFectamine (Life Technologies). After 7 days of expression, supernatants were harvested and the on-rate (ka), off-rate (kd) and affinity constant (KD) of mouse IgG against human TNFR2 was estimated on a BIAcore T200 applying a BIAcore 1:1 binding model except 4G3, 3C11, 3A9 and 3F7 which were fitted using a bivalent model and affinity estimates derived from ka1 and kd1. Blue diamonds represent blocking antibodies, red squares represent non-blockers and black circles were antibodies where blocking data could not be reliably generated.