Table 4. Summary of antibody reagents used for identification of IgG⁺ antigen-specific rabbit B cells.

Marker/Reagent	Cell type	Fluorophore
IgG	Memory B-cells	PE
Antigen	Ag specific B-cell	Alexa-Fluor 488
Antigen	Ag specific B-cell	Alexa-Fluor 647
IgM	Naïve B-cells	PE-Cy7
CD4	T-cells	PerCP
CD8	T-cells	PerCP
Pan-T cell	T cells	PerCP
7AAD	Dead cells

An optimised panel of fluorophore-labelled reagents was developed to maximise the potential of identifying the IgG⁺ antigen-specific subset of B cells in rabbit PBMC samples. Both B cell-specific and non-B cell specific (dump channel) reagents were employed.