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. 2015 Dec 23;7(2):1084–1095. doi: 10.18632/oncotarget.6744

Figure 4. KCs isolated from old mice could attract more MSCs in vitro due to more inflammatory chemokines secretion.

Figure 4

A., Identification of KCs isolated from mice liver. (a)Phenotype of KCs isolated from mice liver; (b) Cytoimmunofluorescence assay was done to identify KCs(CD68, green; nuclei, blue). (400×) B., Identification of MSCs isolated from mice bone marrow. (a), Phenotype of MSCs; (b), MSCs under fluorescent microscope; (c), Oil red O staining after adipogenesis differentiation; (d), Alizarin Red S staining after osteogenesis differentiation. (200×)C., Transwell migration assay was performed to detect MSCs migration to KCs. Migrated MSCs were stained by crystal violet. (200×) D., The quantification of migrated MSCs of C. E., Cytokines concentration in conditioned medium of KCs was detected by bioplex assay. Y-KCs, KCs from young mice; O-KCs, KCs from old mice; Y, young; O, old; Y+C, young+CCl4; O+C, old+CCl4. *P < 0.05.