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A. Autophagy activation in KCs was detected by staining ATG7 by immunofluorescence assay. ATG7 was presented by green fluorescence, DAPI was used to stain nuclei(400×). B. LC3 I/II was tested by western blotting assay. Y, young; O, old; Y+C, young+CCl4; O+C, old+CCl4.
