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. 2015 Nov 22;7(2):1300–1310. doi: 10.18632/oncotarget.6360

Figure 1. The Three-Step-Transcriptional Amplification system provides strong amplification of the PCA3 promoter activity.

Figure 1

A. Scheme of non-replicative reporter adenoviruses. B. The amplification provided by the 3STA was significantly higher than that of the TSTA. PCa cells (22Rv1, LAPC4, PC-3, DU145) were infected with PCA3-fl, PCA3-TSTA, or PCA3-3STA. Cells were harvested after 72 h of infection. The relative fl activity (RLU) was normalized by protein content in each well divided by the SV40 promoter activity in each cell line (RLU = (RLU/μg protein) ÷ (RLU SV40/μg)*100). Each data represents triplicates ± SD; *p < 0.05, **p < 0.01.