Figure 3. PCA3-3STA provided reporter expression levels in vivo comparable to those of the PSEBC-TSTA system.

22Rv1 and LAPC4 PCa cell subcutaneous xenografts were generated in SCID beige mice. Following tumor growth and mice randomization for tumor volume, 10⁸ ivp of either PCA3-3STA or PSEBC-TSTA were injected intratumorally. After 96 h, reporter activity was assessed by bioluminescence. Average photon emission per tumor was measured in 22Rv1 and LAPC4 mouse xenografts (n = 3). No statistically significant difference was observed between the PCA3-3STA and PSEBC-TSTA fl activity in the two cell lines tested.