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. 2015 Nov 29;7(2):1500–1515. doi: 10.18632/oncotarget.6430

Figure 2. p65 localization and binding to IL8 and CCL2 promoters after dl922-947 treatment.

Figure 2

A. 8505-c and BHT101-5 cells were treated with dl922-947 (5 or 1 pfu/mL, respectively). After 24 hours, p65 binding to IL8 (upper panels) and CCL2 (lower panels) promoters was assessed by chromatin immunoprecipitation (ChIP) assay. For each experimental condition, p65 binding is expressed as fold enrichment over the non-specific binding (IgG) control. The results are the mean of three independent experiments ±SEM. One-way ANOVA and Tuckey post-test: ***p<0.001. B. 8505-c and BHT101-5 cells were treated for 24 hours with dl922-947 (5 and 1 pfu/cell, respectively). Cellular fractionation was performed to isolate nuclear and cytosolic fraction. Lysates were probed with p65 antibody, as well as Lamin A/C and GAPDH antibodies to verify the purity of the preparations. The blots are representative of three independent experiments.