Skip to main content
. 2015 Dec 1;7(2):1584–1597. doi: 10.18632/oncotarget.6444

Figure 3. miR-382 promotes the proliferation and cell growth of human HL7702 liver cells.

Figure 3

A. qRT-PCR analysis for miR-382 level in HL7702 cells transfected with miR-382 mimics (miR-382-m), miR-382 inhibitor (miR-382-i), or their respective negative controls (nc-m or nc-i) (n=5). CCK-8 cell counting (n=10) B., EdU (green) cell proliferation assay (n=5) C., and Ki67 (red) immunostaining (n=5) D. demonstrated that miR-382 mimics promoted while miR-382 inhibitor reduced HL7702 cell proliferation. Nuclei were counterstained with Hoechst (blue). Original magnification 100x, scale bar = 50 μm. E. Flow cytometry showed that miR-382 mimics induced a G1 to S phase transition of the cell cycle of HL7702 cells, while miR-382 inhibitor had inverse effect (n=5). *, P<0.05; **, P<0.01.