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. 2015 Nov 30;7(2):1754–1764. doi: 10.18632/oncotarget.6434

Figure 5. PP1α can dephosphorylate AR degrading E3 ligases.

Figure 5

A. HeLa cells were co-transfected with HA-AR, Flag-PP1α and HA-tagged MDM2 or SKP2 as indicated. Cells were then incubated for overnight in androgen-depleted medium for blotting. B. HeLa and LNCaP cells in androgen-depleted medium were treated for 3 hrs with 200 nM of tautomycin, followed by protein normalization for blotting. C. LNCaP cells in androgen-depleted medium were treated for 3 hrs with tautomycin as indicated. Total proteins were normalized for blotting. D. HeLa cells were transfected of Flag-PP1 and HA-MDM2 (left panel) or SKP2 (right panel), without or with AR as indicated. Cells were then incubated for overnight in androgen-depleted medium, followed by anti-Flag Co-IP and blotting.