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. 2015 Nov 30;7(2):1754–1764. doi: 10.18632/oncotarget.6434

Figure 6. Androgen ablation increases PP1α-dependence for AR protein expression and transactivation in PCa cells.

Figure 6

A. C4–2 cells in androgen-depleted medium were treated with tautomycin and androgen (1nM DHT) for qRT-PCR analysis of PSA expression (24 hr treatment) or for cell proliferation (3-day treatment), with the results being normalized to the no tautomycin levels in the absence and presence of DHT. Veh: vehicle; DHT: 1 nM of DHT. B, C. LNCaP and C4–2 cells in androgen-containing medium (10% FBS) were treated with enzalutamide (ENZ, 10 μM) and tautomycin (100, 200, and 400 nM) as indicated. Total proteins (for 24 hr treatment) were harvested and normalized for blotting (B) and total RNA (for 24 hr treatment) was isolated for qRT-PCR analysis of PSA expression (C). The cell proliferation analysis was carried out after 3-day treatment (C). The gene expression and cell growth results were normalized to no tautomycin in the absence and presence of enzalutamide (C). Veh: vehicle; Enz: 10 μM of enzalutamide.