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. 2015 Nov 28;7(2):1765–1776. doi: 10.18632/oncotarget.6426

Figure 1. Glc and Gln are both required to support leukemia cell growth.

Figure 1

Leukemia cells (HL-60, K-562 and THP-1) were exposed to medium containing either glucose or glutamine or both. (A) Time course of cell growth (n = 3). Representative flow cytometry (B) histograms of Ki-67 labelled-HL-60 cells and (C) dot plots for Annexin V/PI labelling of HL-60 cells treated as indicated for 48 hours; these experiments were repeated twice with similar results. Bar graphs represent (D) the glucose consumption and (E) the lactate release (%, normalized per cell number) in HL-60 cells deprived or not of Gln for 48 hours (n = 3). (F) Representative graphs of OCR outputs from the Seahorse analyzer of HL-60 cells treated as indicated (n = 6).