Figure 5. PHGDH expression allows to reduce the extent of oxidative stress induced upon Gln withdrawal.

Bar graphs represent the amounts of total glutathione in HL-60 leukemia cells exposed to a medium (A) with or without Gln (for 6 hours) and (B) with or without exogenous serine (for 48 hours) (n = 3). (C) ROS production (as determined using the H₂- DCFDA probe) in HL-60 leukemia cells exposed for 6 hours to a medium with or without Gln (n = 4). Effects of H₂O₂ (10 μM, 48 hours) on (D) the expression of PHGDH in HL-60 cells and (E) on the growth of HL-60 cells transfected with control or PHGDH shRNA and treated with L-asparaginase.