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. 2015 Dec 21;7(2):2080–2092. doi: 10.18632/oncotarget.6712

Figure 5. Knockdown of ATF6 enhances radiation-induced cell death in glioblastoma.

Figure 5

A. Annexin V/PI assays of D54, LN428 and LN827. Cells were treated with siRNA targeting ATF6 for 48h prior to irradiation with 3 Gy. Cells were stained with Annexin V and PI 96h after irradiation, and analyzed by flow cytometry. B. Cleaved PARP assay in D54. Cells were treated with ATF6 siRNA for 48h prior to irradiation with 3 Gy, and analyzed 96h later by flow cytometry after staining with anti-cleaved PARP-PE antibody. C. Caspase 3/7 activity assay in D54. Cells were treated with ATF6 siRNA for 48h prior to irradiation with 3 Gy, and analyzed 48h later by micro-plate reader D. qRT-PCR analysis of BCL2, BCL-XL, MCL1, and Survivin gene expression in D54 after knockdown of ATF6. In all graphs, data shown are the Means ±SD (n = 3). **** P < 0.0001.