Skip to main content
. 2015 Dec 12;7(2):2093–2104. doi: 10.18632/oncotarget.6573

Figure 2. AEG-1 knockdown inhibited tumor metastasis in vivo.

Figure 2

A. Representative BLI images of athymic nude mice bearing Scc25/AEG-1-siRNA tumors with metastatic lesions. Mice (n = 25) were imaged 6 weeks after tumor cell injection to observe local tumor growth and metastasis. B. Number of metastatic nodules or distant metastasis in individual dead mice bearing control cell or Scc25/AEG-1-siRNA tumors according to H&E-staining results. C. AEG-1 knockdown in Scc25 cells suppressed EMT in mice. H&E staining showed primary tumors with detectable metastases in control mice and without metastases in mice bearing Scc25/AEG-1-siRNA tumors 6 weeks after injection (magnification, ×200). Immunohistochemistry revealed that AEG-1 knockdown decreased vimentin expression and increased E-cadherin staining (magnification × 200).