Table 2. Expression stability values of twelve reference genes as calculated by the NormFinder in P. praeruptorum.
Rank | PEG | NaCl | CuSO4 | H2O2 | MeJA | SA | Cold | Hot | Tissue | Total |
---|---|---|---|---|---|---|---|---|---|---|
1 | PTBP1 | SAND | UBC9 | ACT2 | SAND | SAND | SAND | ACT2 | SAND | SAND |
0.17 | 0.061 | 0.167 | 0.076 | 0.215 | 0.085 | 0.091 | 0.242098 | 0.288 | 0.463 | |
2 | PP2A | TIP41 | PTBP1 | NCBP20 | PP2A | TUB6 | PTBP1 | PTBP1 | EF-1α | ACT2 |
0.189 | 0.15 | 0.193 | 0.215 | 0.253 | 0.18 | 0.197 | 0.263187 | 0.367 | 0.556 | |
3 | NCBP20 | ACT2 | GAPDH | UBC9 | UBC9 | UBC9 | UBC9 | TIP41 | NCBP20 | PTBP1 |
0.349 | 0.245 | 0.221 | 0.238 | 0.308 | 0.243 | 0.22 | 0.340158 | 0.435 | 0.566 | |
4 | CYP2 | UBC9 | ACT2 | PP2A | PTBP1 | PP2A | NCBP20 | PP2A | eIF-4α | CYP2 |
0.357 | 0.539 | 0.234 | 0.25 | 0.315 | 0.275 | 0.231 | 0.456 | 0.44 | 0.622 | |
5 | UBC9 | PP2A | PP2A | PTBP1 | NCBP20 | EF-1α | eIF-4α | eIF-4α | CYP2 | PP2A |
0.372 | 0.61 | 0.329 | 0.308 | 0.35 | 0.331 | 0.237 | 0.472 | 0.494 | 0.677 | |
6 | SAND | GAPDH | CYP2 | EF-1α | eIF-4α | CYP2 | PP2A | CYP2 | ACT2 | UBC9 |
0.467 | 0.681 | 0.439 | 0.328 | 0.363 | 0.362 | 0.26 | 0.479 | 0.568 | 0.746 | |
7 | EF-1α | EF-1α | NCBP20 | SAND | TIP41 | ACT2 | EF-1α | UBC9 | UBC9 | NCBP20 |
0.595 | 0.737 | 0.502 | 0.364 | 0.56 | 0.375 | 0.399 | 0.544 | 0.611 | 0.76 | |
8 | TIP41 | CYP2 | TIP41 | TUB6 | EF-1α | NCBP20 | CYP2 | SAND | PTBP1 | TIP41 |
0.643 | 0.758 | 0.528 | 0.368 | 0.659 | 0.4 | 0.409 | 0.55 | 0.643 | 0.785 | |
9 | eIF-4α | PTBP1 | SAND | eIF-4α | TUB6 | PTBP1 | ACT2 | GAPDH | TUB6 | EF-1α |
0.649 | 0.837 | 0.616 | 0.37 | 0.7 | 0.428 | 0.49 | 0.668 | 0.655 | 0.832 | |
10 | TUB6 | eIF-4α | EF-1α | GAPDH | ACT2 | GAPDH | GAPDH | EF-1α | PP2A | eIF-4α |
0.725 | 1.09 | 0.627 | 0.383 | 0.727 | 0.486 | 0.595 | 0.862 | 0.692 | 0.873 | |
11 | ACT2 | NCBP20 | TUB6 | TIP41 | CYP2 | TIP41 | TIP41 | NCBP20 | TIP41 | TUB6 |
0.792 | 1.607 | 0.742 | 0.485 | 0.804 | 0.512 | 0.634 | 1.021 | 0.907 | 0.932 | |
12 | GAPDH | TUB6 | eIF-4α | CYP2 | GAPDH | eIF-4α | TUB6 | TUB6 | GAPDH | GAPDH |
1.234 | 1.686 | 0.981 | 0.578 | 1.253 | 1.444 | 0.857 | 1.183 | 1.179 | 1.18 |