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. 2016 Mar 29;11(3):e0152510. doi: 10.1371/journal.pone.0152510

Fig 1. Transcript expression analysis of phist and resa-like genes during P. falciparum intraerythrocytic stages.

Fig 1

Gene-specific transcript levels for select phist genes A) and for the resa-like genes B) were analyzed by real time PCR using cDNA prepared from intraerythrocytic synchronized asexual and gametocyte P. falciparum cultures. Note that resa (PFA0110w) and resa2 (PF11_0509) are highly expressed relative to the other resa-like genes. Transcript expression was normalized to the expression of the control gene arginyl tRNA synthetase (PFL0900c). Hours post-synchronization indicate time in hours after adding purified schizonts to fresh blood culture. In the schematic depiction of phist and resa-like genes, yellow represents the signal sequence, and red represents the PEXEL/HT motif. The life cycle stage percentage in the population for each time point is shown in C). The letter “G” indicates mature gametocytes.