Table 1. The primers used to genotype the MSP-1, MSP-2 and GLURP polymorphic regions of P. falciparum isolates from Kalabakan and Kota Marudu, Sabah.

Gene/ Family	Primer	PCR round	Primer sequence
MSP-1	MI-OF	Primary	5’ CTAGAAGCTTTAGAAGATGCAGTATTG 3’
	MI-OR		5’ CTTAAATAGTATTCTAATTCAAGTGGATCA 3’
K1	MI-KF	Nested	5’ AAATGAAGAAGAAATTACTACAAAAGGTGC 3’
	MI-KR		5’ GCTTGCATCAGCTGGAGGGCTTGCACCAGA 3’
MAD20	MI-MF		5’ AAATGAAGGAACAAGTGGAACAGCTGTTAC 3’
	MI-MR		5’ ATCTGAAGGATTTGTACGTCTTGAATTACC 3’
RO33	MI-RF		5’ TAAAGGATGGAGCAAATACTCAAGTTGTTG 3’
	MI-RR		5’ CATCTGAAGGATTTGCAGCACCTGGAGATC 3
MSP-2	M2-OF	Primary	5' ATGAAGGTAATTAAAACATTGTCTATTATA 3'
	M2-OR		5' CTTTGTTACCATCGGTACATTCTT 3'
FC27	M2-FCF	Nested	5' AATACTAAGAGT GTAGGTGCArAT GCTCCA 3'
	M2-FCR		5' TTTTATTTGGTGCATTGCCAGAACTTGAAC 3'
3D7	M2-1CF		5' AGAAGTATGGCAGAAAGTAAkCCTyCTACT 3'
	M2-1CR		5' GATTGTAATTCGGGGGATTCAGTTTGTTCG 3'
GLURP	G-OF	Primary	5’ TGAATT GAAGATGTTCACACTGAAC 3'
	G-OR		5' GTGGAATTGCTTTTTCTTCAACACTAA 3'
	G-OR	Nested	5’ GTGGAATTGCTTTTTCTTCAACACTAA 3’
	G-NF		5’ TGTTCACACTGAACAATTAGATTTAGATCA 3’