Table 2. The primers used to amplify the microsatellite markers of P. falciparum isolates from Kalabakan and Kota Marudu, Sabah.
Chromosome | Locus | Primer sequence (5' to 3') | Size range (bp) |
---|---|---|---|
4 | Poly α-R | ATCAGATAATTGTTGGTA | 114–201 |
Poly α-F | AAAATATAGACGAACAGA | ||
Poly α-3(IR) | (HEX)GAAATTATAACTCTACCA | ||
12 | PFPK2-3R | CCTCAGACTGAAATGCAT | 159–192 |
PFPK2-F | CTTTCATCGATACTACGA | ||
PFPK2-R | (HEX)AAAGAAGGAACAAGCAGA | ||
5 | TA81-3F | GAAGAAATAAGGGAAGGT | 112–142 |
TA81-R | TTTCACACAACACAGGATT | ||
TA81-F | (HEX)TGGACAAATGGGAAAGGATA | ||
11 | ARA2-3(F) | GTACATATGAATCACCAA | 63–90 |
ARA2-R | GCTTTGAGTATTATTAATA | ||
ARA2-F | (HEX)GAATAAACAAAGTATTGCT | ||
6 | TA87-3F | ATGGGTTAAATGAGGTACA | 90–126 |
TA87-R | ACATGTTCATATTACTCAC | ||
TA87-F | (6FAM)AATGGCAACACCATTCAAC | ||
5 | TA42-3F | ACAAAAGGGTGGTGATTCT | 182–251 |
TA42-R | GTATTATTACTACTACTAAAG | ||
TA42-F | (6FAM)TAGAAACAGGAATGATACG | ||
10 | 2490-3R | ATGATGTGCAGATGACGA | 78–93 |
2490-F | TTCTAAATAGATCCAAAG | ||
2490-R | (HEX)TAGAATTATTGAATGCAC | ||
6 | TA1-3(F) | CTACATGCCTAATGAGCA | 159–204 |
TA1-R | TTTTATCTTCATCCCCAC | ||
TA1-F | (6FAM)CCGTCATAAGTGCAGAGC | ||
13 | TA60-F | CTCAAAGAAAAATAATTCA | 69–99 |
TA60-R | AAAAAGGAGGATAAATACAT | ||
TA60-3(IF) | (6FAM)TAGTAACGATGTTGACAA | ||
12 | PFG377-3R | TTATGTTGGTACCGTGTA | 89–113 |
PFG377-F | GATCTCAACGGAAATTAT | ||
PFG377-R | (6FAM)TTATCCCTACGATTAACA |