Table 2.

Comparison of the codon usage for native (VHb) and synthetic (VHb-opt) targeted genes  at Pichia pastoris for expression.

AA	Codon	Host fraction	VHb	VHb-opt
Gly	GGG	0.10	0	0
	GGA	0.32	1	0
	GGT	0.44	7	8
	GGC	0.14	0	0
Glu	GAG	0.43	7	1
	GAA	0.57	2	8
Asp	GAT	0.58	2	8
	GAC	0.42	6	0
Val	GTG	0.19	0	0
	GTA	0.15	0	0
	GTT	0.42	4	10
	GTC	0.23	10	4
Ala	GCG	0.06	0	0
	GCA	0.23	0	0
	GCT	0.45	17	23
	GCC	0.26	6	0
Arg	AGG	0.16	0	0
	AGA	0.48	1	2
	CGG	0.05	0	0
	CGA	0.10	0	0
	CGT	0.16	1	0
	CGC	0.05	0	0
Lys	AAG	0.53	9	10
	AAA	0.47	1	0
Ser	AGT	0.15	0	0
	AGC	0.09	0	0
	TCG	0.09	0	0
	TCA	0.19	0	0
	TCT	0.29	0	1
	TCC	0.20	1	0
Stop	TAA	0.53	1	1
Asn	AAT	0.49	0	0
	AAC	0.51	4	4
Met	ATG	1.00	3	3
Ile	ATA	0.19	0	0
	ATT	0.50	5	5
	ATC	0.30	7	7
Thr	ACG	0.11	0	0
	ACA	0.24	0	0
	ACT	0.40	1	8
	ACC	0.25	7	0
Trp	TGG	1.00	1	1
Cys	TGT	0.65	1	1
	TGC	0.35	0	0
Tyr	TAT	0.46	0	0
	TAC	0.55	4	4
Leu	TTG	0.33	14	14
	TTA	0.16	0	0
	CTG	0.16	0	0
	CTA	0.11	0	0
	CTT	0.16	0	0
	CTC	0.08	0	0
Phe	TTT	0.54	0	2
	TTC	0.46	4	2
Gln	CAG	0.39	6	2
	CAA	0.61	3	7
His	CAT	0.57	1	4
	CAC	0.43	3	0
Pro	CCC	0.15	0	0
	CCG	0.09	0	0
	CCA	0.41	6	7
	CCT	0.35	1	0