Fig. 4.

Qualitative capillary assays demonstrating complementation of the mcpC deletion. Responses of P. putida F1 (pRK415) (WT), XLF004 (pRK415) (ΔmcpC) and XLF004(pXLF204) (ΔmcpC containing mcpC) are shown. Nicotinic acid was provided at 50 mM. Also shown are positive control responses of each strain to 2 % Casamino acids. No response was detected when only chemotaxis buffer was present in the capillary (not shown). Assays were repeated at least three times and representative photographs are shown. Photographs were taken after 7 min.