Fig. 3.
(a) SscI-derived AHLs stimulate ssaI expression and the sscI mutant is decreased for motility. Promoter fusion of ssaI with lacZ reporter (pEC116) paired with Plac-ssaR (pEC116) (Zan et al., 2012) or empty vector (pBBR1-MCS5; Kovach et al., 1995) were transformed into A. tumefaciens NTL4. β-Galactosidase assay was used to monitor the lacZ activity with and without organic extracts. Error bars represent the standard deviation from three replicates. (b) sscI is involved in motility control. WT KLH11 and derivatives were inoculated on MB 2216 (supplemented with 0.25 %, w/v, agar) swim agar plates for 1 week at 28 °C. The results shown are representatives of several independent experiments each with three biological replicates.