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β-Galactosidase assay of the expression of an rpaI-lacZ fusion. Rh. palustris CGA814 was used as the reporter strain. Organic extracts of KLH11 strains were prepared from cultures grown in MB 2216 supplemented with 1 mM para-coumarate. An extract of MB 2216 plus para-coumarate was used as the negative control and 1 µM pC-HSL was used as the positive control. Bars represent the mean of three biological replicates and the error bars represent the sd of triplicates.
