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CLSM micrographs of biofilms of P. fluorescens (a, b) SBW25 Tn7::gfp2 and (c, d) SBW25 Tn7::gfp2ΔviscA. The strains were cultivated in flow chamber systems for 1 day with AB minimal medium with citrate, upon which a shift was made to AB minimal medium with no carbon source. Micrographs were acquired immediately before the shift (a, c) and 3 h after the shift (b, d). Bars, 20 μm.
