Table 1. Bacterial strains and plasmids used in this study.
| Strain or plasmid * | Relevant genotype† or description | Source/reference |
|---|---|---|
| C. jejuni | ||
| M1 | WT | Friis et al. (2010) |
| M1 coupled to ΔflaB | WT | This study |
| M1 coupled to ΔflaB*, ΔflaA, ΔflaAB | WT | This study |
| M1 coupled to ΔflaB+empty GC | WT; Kmr | This study |
| M1 coupled to ΔflaD, ΔpflA | WT | This study |
| M1 coupled to ΔfliW | WT | This study |
| M1 coupled to ΔfliW+empty GC | WT; Kmr | This study |
| M1 ΔflaB clones A, B, C, D, E | ΔCJM1_1294; Cmr | This study |
| M1 ΔflaB* clones A, B, C | ΔCJM1_1295-1294; Cmr | This study |
| M1 ΔflaA clones A, B, C | ΔCJM1_1296; Cmr | This study |
| M1 ΔflaAB clones A, B, C | ΔCJM1_1295-1294/CJM1_1296; Cmr | This study |
| M1 ΔflaD clones A, B, C | ΔCJM1_0851; Cmr | This study |
| M1 ΔpflA clones A, B, C | ΔCJM1_1501; Cmr | This study |
| M1 ΔfliW clone A, B, C | ΔCJM1_1052; Cmr | This study |
| M1 ΔfliW clone A+empty GC | ΔCJM1_1052; Cmr Kmr | This study |
| M1 ΔfliW clone A+fliW GC | ΔCJM1_1052+CJM1_1052; Cmr Kmr | This study |
| M1 ΔflaB clone D+empty GC | ΔCJM1_1294; Cmr Kmr | This study |
| M1 ΔflaB clone D+fliW GC | ΔCJM1_1294+CJM1_1052; Cmr Kmr | This study |
| M1 ΔflaB clone E+empty GC | ΔCJM1_1294; Cmr Kmr | This study |
| M1 ΔflaB clone E+fliW GC | ΔCJM1_1294+CJM1_1052; Cmr Kmr | This study |
| E. coli strains | ||
| NEB 10-β or 5-α | Host strains for pSV009 and derivatives thereof | New England Biolabs |
| Plasmids | ||
| pCC027 | Cmr donor plasmid | Coward et al. (2008) |
| pMiniT | E. coli PCR cloning plasmid | New England Biolabs |
| pUC19 | E. coli cloning plasmid | New England Biolabs; Yanisch-Perron et al. (1985) |
| pSV009 | C. jejuni genetic complementation plasmid, pUC19 backbone; donor for empty GC region | This study |
| pSV012 | pSV009 containing fliW; donor for fliW GC region | This study |
*
empty GC, Empty genetic complementation region amplified from pSV009; fliW GC, genetic complementation region containing the fliW promoter and coding sequence amplified from pSV012.
†
Cmr, chloramphenicol resistance; Kmr, kanamycin resistance.