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. 2015 Dec;161(Pt 12):2434–2443. doi: 10.1099/mic.0.000194

Fig. 2.

Fig. 2.

Influence of LeuO on the cadC promoter. (a) E. coli containing the cadC-lacZ reporter plasmid pXB239 and the pBAD33-leuO plasmid pVA126 was grown in LB broth in the presence or absence of 0.02 % arabinose for 5 h when β-galactosidase activity was determined. The presented data are the mean ± sd of three independent experiments. Statistical significance was determined using a t-test comparing the mean of the induced strain with the mean of 0 % arabinose control; *P < 0.005. (b) Gel shift assays were performed using purified LeuO-MBP or MBP and the two indicated DNA fragments from the cadC promoter. Nucleotide numbering listed for the cadC1 and cadC2 DNA fragments are relative to the cadC transcriptional start site. Biotin-labelled cadC1 or cadC2 DNA fragments (1.5 nM) were incubated with either purified LeuO-MBP or MBP at 0 μM (lane 1), 10 μM (lane 2), 20 μM (lane 3) or 30 μM (lane 4) prior to electrophoresis.